The Transgenic Core Facility (TCF) of the IRC was established in 2004 to cater to the needs of IRC investigators. The mission of the TCF is to provide efficient and economic access to transgenic animals and related technologies.
'The Transgenic Core Facility (TCF) was established in 2004 to offer different transgenic-related services to the research groups of the IRC. At the same time, we provide our expertise to other research groups of the VIB and several research labs of the UGent.
Our main activities include:
- The design of gene targeting constructs, designer nucleases and generation of genetically engineered ES cells
- Generation of genetically modified mice via microinjection of DNA/RNA constructs into zygotes or blastocyst injection of targeted ES cells
- Rederivations of mouse strains via embryo transfer and/or in vitro fertilization (IVF)
- Archiving of different transgenic mouse lines via embryo and sperm cryopreservation
The Transgenic Core Facility uses the mouse as model organism. Over the years several technologies have been developped to engineer the mouse genome, such as cDNA/BAC injections, ES-cell targeting and CRISPR/Cas9 technology. Sperm and embryos of mutant mouse lines are also relatively easy to cryopreserve.'
To showcase the world-class scientific research of the Transgenic Core Facility, you can discover their scientific papers in more detail.
We are always on the lookout for highly motivated colleagues to join our team. If you are interested, please contact us.
The Transgenic Core Facility can only thrive thanks to the dedication and commitment of its people, no matter what their function or seniority.
To stay up to date in rapidly developing fields, scientists regularly interact with (international) colleagues. Conferences and other (scientific) events are an excellent way to facilitate such a continent-spanning knowledge exchange.