Laboratory of Advanced Live Cell Imaging
The discovery of fluorescent proteins allowed monitoring intracellular processes in real time and brought dynamic behaviour and transitions as novel parameters, measurable by microscopic imaging. The advanced live cell-imaging group is cornered on the use and the development of various tools to optimize dynamic imaging of plant intracellular processes.
The major research topic in the group aims to elucidate the highly dynamic process of endocytosis at the plasma membrane in plant cells. Endocytosis is a stepwise process where several players are recruited in a highly specific order. Following the recruitment of early adaptor proteins, which recognize transmembrane cargo proteins such as receptors and channels destined for internalization, endocytosis progresses through the formation of a coated pit. Fission of the formed vesicle subsequently allows the physical internalization of the membrane proteins together with their associated ligands and lipids.
Towards mechanistic insight into the TPLATE adaptor complex in plant endocytosis
Developing proximity biotinylation assays in plants to expand the interactomics toolbox
Visualizing differential temporal recruitment of the endocytic players at the PM
Developing conditional knockout strategies at the protein level
Generating structural and biochemical insight into multi-subunit complexes
Visualizing protein-protein interactions
Unravelling the link between endocytosis and autophagy-mediated degradation.
To showcase the world-class scientific research of the Daniël Van Damme Lab, you can discover their scientific papers in more detail.
We are always on the lookout for highly motivated colleagues to join our team. If you are interested to work on the interplay between cell and structural biology, please contact us.
The Daniël Van Damme Lab can only thrive thanks to the dedication and commitment of its people, no matter what their function or seniority.
To stay up to date in rapidly developing fields, scientists regularly interact with (international) colleagues. Conferences and other (scientific) events are an excellent way to facilitate such a continent-spanning knowledge exchange.