The VIB Protein Core offers solutions for producing mg amounts of research grade proteins, or to optimize expression vectors and production conditions in different systems. When the pure protein is not the endpoint, also Conjugation of the purified protein is possible.

Research areas

Computational biology Immunology & inflammation Medical biotechnology Microbiology Structural biology Systems biology

Research focus

The VIB Protein Core offers solutions for producing mg amounts of research grade proteins, or to optimize expression vectors and production conditions in different systems. When the pure protein is not the endpoint, also Conjugation of the purified protein is possible.

The Protein Core services include scale up of microbial cultures up to 6 or 20 L, mammalian cell culture up to 12 L, and downstream processing and purification adapted to those scales. The deliverable is the end product of your process (usually a protein) and a documentation that each step is carried out to the state of the art. In order to rapidly optimize expression analysis, the VIB Protein Core uses a technology platform for parallel expression analysis with numerous possible combinations, i.e. FastScreen. Expression optimization is offered in Escherichia coli, Pichia pastoris and mammalian cells (HEK293F). Matrix-approach based optimizations are also available for refolding of inclusion bodies, i.e. FastFold, and for purification process optimization, i.e. FastClean.

Based on the FastScreen result, a Production can be performed. The FastClean can lead to a Purification strategy to make mg amounts of pure protein. Depending on the purpose of the pure protein; fluorescent labels, polymers, … can be attached by Conjugation. Protein Analytics can be performed to guarantee the quality of the delivered protein. Some Protein Analytics are done in house, others in collaboration with specialized groups or institutes. Not only the purity, concentration or LPS content is important but also the molecular weight in solution of the protein (measured by SEC/MALS) and the activity or affinity. Therefor the Protein Core performs also different (cellular) Activity Assays and invests in technologies for Protein-Protein Interactions.

Beyond servicing, Research on new technologies and opportunities available on the market is done regularly by the VIB Protein Core. By the enormous interest in single domain antibodies (also called nanobodies®), the VIB Protein Core has specialized in Nanobody® Production and Purification.

 

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Publications

To showcase the world-class scientific research of the VIB Protein Core, you can discover their scientific papers in more detail.

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Jobs

We are always on the lookout for highly motivated colleagues to join our team. If you are interested to work in this lab please contact us.

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Team

The VIB Protein Core can only thrive thanks to the dedication and commitment of its people, no matter what their function or seniority. 

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Events

To stay up to date in rapidly developing fields, scientists regularly interact with (international) colleagues. Conferences and other (scientific) events are an excellent way to facilitate such a continent-spanning knowledge exchange.

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